Scientists in Spain have received funding to test whether an extinct
mountain goat can be cloned from preserved cells. The bucardo became
extinct in 2000, but cells from the last animal were frozen in liquid
nitrogen.
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A Spanish mountain goat, the Pyrenean Ibex, was formally confirmed as extinct in 2000
when the last one of its kind was discovered dead [Credit: Gobierno de Aragon] |
In 2003, a cloned calf was brought to term but died a few minutes after
birth. Now, the scientists will test the viability of the female
bucardo's 14-year-old preserved cells.
The bucardo, or Pyrenean ibex, calf born through cloning was an historic
event: the first "de-extinction", in which a lost species or
sub-species was resurrected.
The Aragon Hunting Federation signed an agreement with the Centre for
Research and Food Technology of Aragon (CITA) in Zaragoza to begin
preliminary work on the cells from the last animal, named Celia.
One of the scientists behind the cloning effort, Dr Alberto
Fernandez-Arias, told BBC News: "At this moment, we are not initiating a
'bucardo recovery plan', we only want to know if Celia's cells are
still alive after having been maintained frozen during 14 years in
liquid nitrogen."
In addition to this in vitro work, they will also attempt to clone embryos and implant them in female goats.
"In this process, one or more live female bucardo clones could be
obtained. If that is the case, the feasibility of a bucardo recovery
plan will be discussed," Dr Fernandez-Arias, who is head of the Aragon
Hunting, Fishing and Wetlands Service, explained.
The bucardo (Capra pyrenaica pyrenaica) was a sub-species of ibex, with
distinct physical and genetic characteristics to other mountain goats
inhabiting the Iberian Peninsula. It was perfectly adapted to life in
its mountain habitat, and to survive the extreme cold and snow of winter
in the Pyrenees.
However, its population had been declining for years for several
reasons, including hunting. In April 1999, researchers captured the last
animal, a female named Celia. They obtained skin biopsies and froze the
tissue in liquid nitrogen at a temperature of -196C (-321F).
The following year, Celia was killed by a falling tree in the National
Park of Ordesa in north-east Spain. But a team including Dr
Fernandez-Arias, Jose Folch and others were able to inject nuclei from
Celia's preserved cells into goat eggs that had been emptied of their
own DNA.
Then they implanted the eggs into surrogates - hybrids between Spanish
ibex and domestic goats. Of 57 implantations, seven animals became
pregnant and one was carried to term.
The baby bucardo was born in 2003 - the first successful
"de-extinction". But the clone of Celia died a few minutes later due to a
defect in one of its lungs. Earlier this year, Dr Fernandez-Arias
related the story in a TEDx talk, as part of a meeting on de-extinction.
Even if the new effort succeeds in producing healthy clones, any future
recovery plan for the bucardo would be fraught with difficulty -
especially given the only frozen tissue is from a lone female.
One possible approach for bringing back the bucardo might be to cross a
healthy female bucardo clone with a closely related sub-species - such
as the Spanish ibex (Capra pyrenaica hispanica) or the Gredos ibex
(Capra pyrenaica victoriae) - and then selectively breeding the
offspring to enhance traits typical of the bucardo.
Several other possibilities could also be explored. For instance,
researchers have been able to reverse the sex of female mouse embryos by
introducing a key gene that makes them develop as males.
Other options
In addition, George Church, professor of genetics at Harvard University,
explained that a technique known as Crispr opened up new opportunities
in the field of endangered species conservation and de-extinction. The
technique allows researchers to edit genomes with extraordinary
precision.
Such "genome editing" techniques could be used to introduce genetic
diversity in populations that are so closely related it poses a threat
to their survival.
"In some cases, you have a hunch as to what diversity is needed. You
might specifically want diversity in the major histocompatibility
complex [a large gene family involved in immune responses]," Prof Church
told BBC News.
"For example, part of the problem with the Tasmanian devil is that they
are so closely related in terms of their immune system that they have
problems rejecting the facial tumour cells that they spread by biting
each other."
However, he said, such techniques might eventually offer a way to
extensively edit the genome of an Asian elephant to make it more like a
mammoth, using a genetic sequence from the extinct animals.
Commenting on plans for the bucardo cells, the Aragon Hunting Federation
said it wanted to "develop initiatives in the field of ecology in order
to defend the natural environment".
The sum provided to fund the research at CITA has not been disclosed.